Faculty Collaboration Database

Mesenchymal stromal cells are present in the heart and promote growth of adult stem cells in vitro. Cytotherapy 2011 Apr;13(4):400-6

Date

11/26/2010

Pubmed ID

21090918

DOI

10.3109/14653249.2010.529890

Scopus ID

2-s2.0-79952667750 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

BACKGROUND AIMS: For many years the human heart has been considered a terminally differentiated organ with no regenerative potential after injury. Recent studies, however, have cast doubt on this long-standing dogma. The objective of this study was to investigate the presence of and characterize mesenchymal stromal cells (MSC) in the adult mouse heart. The impact of MSC on growth and differentiation of adult cardiac stem cells (CSC) was also analyzed.

METHODS: A combination of lineage-negative/c-kit-negative (Lin(-)/c-kit(-)) immunoselection with a plastic-adhesion technique was used to isolate cardiac-derived MSC. The differentiation capacity and expression of surface markers were analyzed. To investigate the impact of MSC on growth and differentiation of adult CSC, Green Fluorescent Protein (GFP(+)) adult CSC were co-cultured with GFP(-) cardiac-derived MSC.

RESULTS: MSC were present in the adult mouse heart and they met the criteria established to define mouse MSC. They expressed surface markers and were able to differentiate, in a controlled manner, into multiple lineages. In addition, cardiac-derived MSC promoted the survival and expansion of adult CSC in vitro.

CONCLUSIONS: MSC can be isolated from the mouse heart and they promote growth and differentiation of adult CSC. The findings from this study could have a significant beneficial impact on future heart failure treatment. Co-culture and co-implantation of cardiac-derived MSC with adult CSC could provide extensive cardiac regeneration and maintenance of the CSC population after implanted into the heart.

Author List

Lushaj EB, Anstadt E, Haworth R, Roenneburg D, Kim J, Hematti P, Kohmoto T

Authors

Peiman Hematti MD Professor in the Medicine department at Medical College of Wisconsin
Takushi Kohmoto MD, PhD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult Stem Cells
Animals
Cell Differentiation
Cells, Cultured
Coculture Techniques
Male
Mice
Mice, Inbred C57BL
Myocardium
Stromal Cells