Quantitative phenotype analysis to identify, validate and compare rat disease models. Database (Oxford) 2019 Jan 01;2019
Date
04/03/2019Pubmed ID
30938777Pubmed Central ID
PMC6444380DOI
10.1093/database/baz037Scopus ID
2-s2.0-85064202973 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
The laboratory rat has been widely used as an animal model in biomedical research. There are many strains exhibiting a wide variety of phenotypes. Capturing these phenotypes in a centralized database provides researchers with an easy method for choosing the appropriate strains for their studies. Existing resources have provided some preliminary work in rat phenotype databases. However, existing resources suffer from problems such as small number of animals, lack of updating, web interface queries limitations and lack of standardized metadata. The Rat Genome Database (RGD) PhenoMiner tool has provided the first step in this effort by standardizing and integrating data from individual studies. Our work, mainly utilizing data curated in RGD, involves the following key steps: (i) we developed a meta-analysis pipeline to automatically integrate data from heterogeneous sources and to produce expected ranges (standardized phenotype ranges) for different strains and phenotypes under different experimental conditions; (ii) we created tools to visualize expected ranges for individual strains and strain groups. We developed a meta-analysis pipeline and an interactive web interface that summarizes and visualizes expected ranges produced from the meta-analysis pipeline. Automation of the pipeline allows for updates as additional data becomes available. The interactive web interface provides curators and researchers with a platform for identifying and validating expected ranges for a variety of quantitative phenotypes. The data analysis result and visualization tools will promote an understanding of rat disease models, guide researchers to choose optimal strains for their research needs and encourage data sharing from different research hubs. Such resources also help to promote research reproducibility. The interactive platforms created in this project will continue to provide a valuable resource for translational research efforts.
Author List
Zhao Y, Smith JR, Wang SJ, Dwinell MR, Shimoyama MAuthors
Melinda R. Dwinell PhD Professor in the Physiology department at Medical College of WisconsinShur-Jen Wang Research Scientist II in the Physiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBlood Pressure
Body Weight
Databases, Genetic
Disease Models, Animal
Female
Genome
Male
Meta-Analysis as Topic
Models, Biological
Phenotype
Publication Bias
Quality Control
Rats
Software
Systole
