Faculty Collaboration Database

Probing Ca²⁺ release mechanisms using sea urchin egg homogenates. Methods Cell Biol 2019;151:445-458

Date

04/06/2019

Pubmed ID

30948025

DOI

10.1016/bs.mcb.2018.10.007

Scopus ID

2-s2.0-85061704699

Abstract

Sea urchin eggs have been extensively used to study Ca²⁺ release through intracellular Ca²⁺-permeable channels. Their amenability to homogenization yields a robust, cell-free preparation that was central to establishing the Ca²⁺ mobilizing actions of cyclic ADP-ribose and NAADP. Egg homogenates have continued to provide insight into the basic properties and pharmacology of intracellular Ca²⁺ release channels. In this chapter, we describe methods for the preparation of egg homogenates and monitoring Ca²⁺ release using fluorimetry and radiotracer flux.

Author List

Yuan Y, Gunaratne GS, Marchant JS, Patel S

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Calcium
Calcium Signaling
Cell-Free System
Cyclic ADP-Ribose
Kinetics
NADP
Ovum
Proteins
Sea Urchins