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Probing Ca2+ release mechanisms using sea urchin egg homogenates. Methods Cell Biol 2019;151:445-458

Date

04/06/2019

Pubmed ID

30948025

DOI

10.1016/bs.mcb.2018.10.007

Scopus ID

2-s2.0-85061704699

Abstract

Sea urchin eggs have been extensively used to study Ca2+ release through intracellular Ca2+-permeable channels. Their amenability to homogenization yields a robust, cell-free preparation that was central to establishing the Ca2+ mobilizing actions of cyclic ADP-ribose and NAADP. Egg homogenates have continued to provide insight into the basic properties and pharmacology of intracellular Ca2+ release channels. In this chapter, we describe methods for the preparation of egg homogenates and monitoring Ca2+ release using fluorimetry and radiotracer flux.

Author List

Yuan Y, Gunaratne GS, Marchant JS, Patel S

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Calcium
Calcium Signaling
Cell-Free System
Cyclic ADP-Ribose
Kinetics
NADP
Ovum
Proteins
Sea Urchins
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a