Probing Ca2+ release mechanisms using sea urchin egg homogenates. Methods Cell Biol 2019;151:445-458
Date
04/06/2019Pubmed ID
30948025DOI
10.1016/bs.mcb.2018.10.007Scopus ID
2-s2.0-85061704699 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Sea urchin eggs have been extensively used to study Ca2+ release through intracellular Ca2+-permeable channels. Their amenability to homogenization yields a robust, cell-free preparation that was central to establishing the Ca2+ mobilizing actions of cyclic ADP-ribose and NAADP. Egg homogenates have continued to provide insight into the basic properties and pharmacology of intracellular Ca2+ release channels. In this chapter, we describe methods for the preparation of egg homogenates and monitoring Ca2+ release using fluorimetry and radiotracer flux.
Author List
Yuan Y, Gunaratne GS, Marchant JS, Patel SAuthor
Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCalcium
Calcium Signaling
Cell-Free System
Cyclic ADP-Ribose
Kinetics
NADP
Ovum
Proteins
Sea Urchins