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Murine aortic reactivity is programmed equally by maternal low protein diet or late gestation dexamethasone. J Matern Fetal Neonatal Med 2007 Nov;20(11):833-41

Date

09/14/2007

Pubmed ID

17853187

DOI

10.1080/14767050701626540

Scopus ID

2-s2.0-35349015382   12 Citations

Abstract

OBJECTIVE: In rats, maternal low protein diet induces growth restriction, increases fetal glucocorticoid exposure and programs cardiovascular and endocrine dysfunction in adult offspring. We hypothesized that both maternal low protein diet and late gestation dexamethasone program murine offspring to develop hypertension, vascular dysfunction, and glucose intolerance.

METHODS: An iso-caloric low protein diet (LP) was provided to dams from E0 to E19. Additional dams received a normal protein diet without (NP) or with either dexamethasone (NP-Dex, 0.1 mg/kg/d sc) or normal saline (NP-NS) from E10 to E18.

RESULTS: Offspring of dams given LP weighed less at 10 days than NP offspring, while Dex administration did not alter pup weight. At 4 months, all four groups had similar systolic blood pressures and no detectable differences were evoked by oral L-NAME. Offspring of LP mice had impaired glucose clearance that was directly correlated with their weight at 10 days. Aortic rings from offspring of both LP and NP-Dex exposed dams had impaired vasodilatation to acetylcholine.

CONCLUSIONS: These findings demonstrate that both maternal low protein diet and late gestation dexamethasone program murine offspring to develop endothelial dysfunction in the absence of hypertension, while only maternal LP impaired perinatal growth and glucose clearance in adult offspring.

KEYWORDS: Acetylcholine; blood pressure; developmental biology; fetal programming

Author List

Roghair RD, Segar JL, Kilpatrick RA, Segar EM, Scholz TD, Lamb FS

Author

Jeffrey L. Segar MD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Newborn
Blood Pressure
Dexamethasone
Diet, Protein-Restricted
Disease Models, Animal
Endothelium
Female
Fetal Development
Glucocorticoids
Glucose Tolerance Test
Male
Mice
Pregnancy
Prenatal Exposure Delayed Effects
jenkins-FCD Prod-478 d1509cf07a111124a2d122fd3df854cc0b993c00