Genetic mapping of a mouse ocular malformation locus, Tcm, to chromosome 4. Mamm Genome 1997 Mar;8(3):178-81
Date
03/01/1997Pubmed ID
9069117DOI
10.1007/s003359900385Scopus ID
2-s2.0-0031089330 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
The Tcm mutation in the mouse is an autosomal dominant ocular malformation manifesting as microphthalmia, iris dysplasia, cataract, and coloboma. As a first step to cloning the Tcm gene, we report the localization of the Tcm mutation with respect to known microsatellite markers. Backcross progeny carrying the Tcm mutation were produced by mating Tcm/+ heterozygous mice to normal C57BL/6 partners. Genomic DNA from each mouse was subjected to PCR analysis to identify simple sequence length polymorphisms. Our results locate Tcm to Chr 4 and suggest candidate genes responsible for the Tcm phenotype. Finally, ocular histopathology was done in 3-week-old animals to define the extent of the malformation.
Author List
Zhou E, Grimes P, Favor J, Koeberlein B, Pretsch W, Neuhauser-Klaus A, Sidjanin D, Stambolian DAuthor
Danielle Sidjanin Maier PhD Nurse Practitioner in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Abnormalities, MultipleAnimals
Chromosome Mapping
Eye Abnormalities
Female
Male
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Polymorphism, Genetic
