An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells. J Cell Biol 2017 Dec 04;216(12):3981-3990
Date
10/13/2017Pubmed ID
29021220Pubmed Central ID
PMC5716285DOI
10.1083/jcb.201704085Scopus ID
2-s2.0-85037711788 (requires institutional sign-in at Scopus site) 28 CitationsAbstract
Human pluripotent stem cells (hPSCs) self-organize into apicobasally polarized cysts, reminiscent of the lumenal epiblast stage, providing a model to explore key morphogenic processes in early human embryos. Here, we show that apical polarization begins on the interior of single hPSCs through the dynamic formation of a highly organized perinuclear apicosome structure. The membrane surrounding the apicosome is enriched in apical markers and displays microvilli and a primary cilium; its lumenal space is rich in Ca2+ Time-lapse imaging of isolated hPSCs reveals that the apicosome forms de novo in interphase, retains its structure during mitosis, is asymmetrically inherited after mitosis, and relocates to the recently formed cytokinetic plane, where it establishes a fully polarized lumen. In a multicellular aggregate of hPSCs, intracellular apicosomes from multiple cells are trafficked to generate a common lumenal cavity. Thus, the apicosome is a unique preassembled apical structure that can be rapidly used in single or clustered hPSCs to initiate self-organized apical polarization and lumenogenesis.
Author List
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DLAuthor
Kenichiro Taniguchi PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ActinsBiomarkers
Calcium
Calnexin
Cell Line
Cell Polarity
Cytokinesis
Cytoskeletal Proteins
Gene Expression
Germ Layers
Humans
Interphase
Lamin Type A
Mitosis
Morphogenesis
Pluripotent Stem Cells
Sequestosome-1 Protein
Single-Cell Analysis
Time-Lapse Imaging
