Faculty Collaboration Database

An innovative in situ method of creating hybrid dendrimer nano-assembly: An efficient next generation dendritic platform for drug delivery. Nanomedicine 2019 Oct;21:102043

Date

06/28/2019

Pubmed ID

31247312

DOI

10.1016/j.nano.2019.102043

Scopus ID

2-s2.0-85068969431 (requires institutional sign-in at Scopus site)   24 Citations

Abstract

Dendrimers have proven to be effective for drug delivery and their biodisposition varies with change on their surface, generation and core. In an effort to understand the role of critical nanoscale design parameters, we developed a novel hybrid dendrimer approach to harness unique features of individual dendrimers and create a nano-assembly. We report an easy in situ method of creating hybrid dendrimer nano-assembly by mixing G4.0 PAMAM (-NH₂) and G3.5 PAMAM (-COONa) dendrimers with a chemotherapeutic drug docetaxel (DTX). Zeta potential, HR-TEM, ¹H-NMR proved the formation of nano-assembly. In vitro dissolution, release studies revealed pH dependent dissolution and sustained drug release. Cellular uptake, cytotoxicity, and flow cytometric analysis in human/mouse glioblastoma cells indicated the effectiveness of hybrid dendrimers. The oral administration of the hybrid dendrimers showed pharmacokinetic equivalence to intravenous injection of commercially available Taxotere®. Hybrid dendrimer concept provides much needed fine-tuning to create multistage next-generation dendritic platform in nanomedicine.

Author List

Singh MK, Kuncha M, Nayak VL, Sarma AVS, Kumar MJM, Chauhan AS, Sistla R

Author

Abhay Chauhan PhD Associate Professor in the School of Pharmacy Administration department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Administration, Oral
Animals
Cell Line, Tumor
Dendrimers
Drug Delivery Systems
Heterografts
Humans
Mice
Nanocomposites
Nanomedicine
Neoplasms
Nylons