Comparison of the Effects of High-Fat Diet on Energy Flux in Mice Using Two Multiplexed Metabolic Phenotyping Systems. Obesity (Silver Spring) 2019 May;27(5):793-802
Date
04/03/2019Pubmed ID
30938081Pubmed Central ID
PMC6478533DOI
10.1002/oby.22441Scopus ID
2-s2.0-85063691148 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
OBJECTIVE: Multiplexed metabolic phenotyping systems are available from multiple commercial vendors, and each system includes unique design features. Although expert opinion supports strengths and weaknesses of each design, empirical data from carefully controlled studies to test the biological impact of design differences are lacking.
METHODS: Wild-type C57BL/6J mice of both sexes underwent phenotyping in OxyMax (Columbus Instruments International) and Promethion (Sable Systems International) systems located within the same room of a newly constructed animal research facility in a crossover design study. Phenotypes were examined under chow (2920×)-fed conditions and again after 4 weeks of 60% high-fat diet (D12492) feeding.
RESULTS: Food intake, physical activity, and respiratory gas exchange data significantly diverged between systems, depending upon sex of animals and diet supplied. Estimates of energy expenditure based on gas exchange in both systems accounted for a fraction of consumed calories that was greater in males than females.
CONCLUSIONS: Design differences quantitatively impact the assessment of metabolic end points and thus the qualitative interpretation of various interventions. Importantly, current multiplexed systems remain blind to multiple additional end points, including digestive efficiency and selected forms of energy flux (nitrogenous, anaerobic, etc.), that account for a physiologically and/or pathophysiologically significant fraction of total energy flux.
Author List
Soto JE, Burnett CML, Ten Eyck P, Abel ED, Grobe JLAuthor
Justin L. Grobe PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsDiet, High-Fat
Energy Intake
Female
Male
Mice
Mice, Inbred C57BL
Phenotype
