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Obesity alters immune and metabolic profiles: New insight from obese-resistant mice on high-fat diet. Obesity (Silver Spring) 2016 10;24(10):2140-9

Date

08/16/2016

Pubmed ID

27515998

Pubmed Central ID

PMC5039085

DOI

10.1002/oby.21620

Scopus ID

2-s2.0-84988699941   44 Citations

Abstract

OBJECTIVE: Diet-induced obesity has been shown to alter immune function in mice, but distinguishing the effects of obesity from changes in diet composition is complicated. It was hypothesized that immunological differences would exist between diet-induced obese (DIO) and obese-resistant (OB-Res) mice fed the same high-fat diet (HFD).

METHODS: BALB/c mice were fed either standard chow or HFD to generate lean or DIO and OB-Res mice, respectively. Resulting mice were analyzed for serum immunologic and metabolic profiles and cellular immune parameters.

RESULTS: BALB/c mice on HFD were categorized as DIO or OB-Res, based on body weight versus lean controls. DIO mice were physiologically distinct from OB-Res mice, whose serum insulin, leptin, gastric inhibitory polypeptide, and eotaxin concentrations remained similar to lean controls. DIO mice had increased macrophage(+) crown-like structures in white adipose tissue, although macrophage percentages were unchanged from OB-Res and lean mice. DIO mice also had decreased splenic CD4(+) T cells, elevated serum GM-CSF, and increased splenic CD11c(+) dendritic cells, but impaired dendritic cell stimulatory capacity (Pa??<a??0.05 vs. lean controls). These parameters were unaltered in OB-Res mice versus lean controls.

CONCLUSIONS: Diet-induced obesity results in alterations in immune and metabolic profiles that are distinct from effects caused by HFD alone.

Author List

Boi SK, Buchta CM, Pearson NA, Francis MB, Meyerholz DK, Grobe JL, Norian LA

Author

Justin L. Grobe PhD Associate Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Body Weight
CD4-Positive T-Lymphocytes
Chemokine CCL11
Diet, High-Fat
Female
Insulin
Leptin
Male
Metabolome
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Obese
Obesity
Spleen