Faculty Collaboration Database

Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection. J Mol Cell Cardiol 2009 Aug;47(2):256-63

Date

03/31/2009

Pubmed ID

19328206

Pubmed Central ID

PMC4329292

DOI

10.1016/j.yjmcc.2009.03.009

Scopus ID

2-s2.0-67649265352 (requires institutional sign-in at Scopus site)   106 Citations

Abstract

Nitric oxide is an important messenger in numerous biological processes, such as angiogenesis, hypoxic vasodilation, and cardioprotection. Although nitric oxide synthases (NOS) produce the bulk of NO, there is increasing interest in NOS independent generation of NO in vivo, particularly during hypoxia or anoxia, where low oxygen tensions limit NOS activity. Interventions that can increase NO bioavailability have significant therapeutic potential. The use of far red and near infrared light (R/NIR) can reduce infarct size, protect neurons from methanol toxicity, and stimulate angiogenesis. How R/NIR modulates these processes in vivo and in vitro is unknown, but it has been suggested that increases in NO levels are involved. In this study we examined if R/NIR light could facilitate the release of NO from nitrosyl heme proteins. In addition, we examined if R/NIR light could enhance the protective effects of nitrite on ischemia and reperfusion injury in the rabbit heart. We show both in purified systems and in myocardium that R/NIR light can decay nitrosyl hemes and release NO, and that this released NO may enhance the cardioprotective effects of nitrite. Thus, the photodissociation to NO and its synergistic effect with sodium nitrite may represent a noninvasive and site-specific means for increasing NO bioavailability.

Author List

Lohr NL, Keszler A, Pratt P, Bienengraber M, Warltier DC, Hogg N

Authors

Neil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of Wisconsin
Agnes Keszler PhD Research Scientist I in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cardiotonic Agents
Electron Spin Resonance Spectroscopy
Free Radical Scavengers
Hemeproteins
Hemodynamics
Hemoglobins
Infrared Rays
Luminescent Measurements
Myocardial Reperfusion Injury
Myoglobin
Nitric Oxide
Ozone
Rabbits
Spectrum Analysis