Faculty Collaboration Database

Osteogenic potential of sol-gel bioactive glasses containing manganese. J Mater Sci Mater Med 2019 Jul 13;30(7):86

Date

07/16/2019

Pubmed ID

31302783

DOI

10.1007/s10856-019-6288-9

Scopus ID

2-s2.0-85069046514 (requires institutional sign-in at Scopus site)   50 Citations

Abstract

Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO₂, 36 mol% CaO, 4 mol% P₂O₅) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mn-containing BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn²⁺ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Results show that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration.

Author List

Barrioni BR, Norris E, Li S, Naruphontjirakul P, Jones JR, Pereira MM

Author

Test W. User test user title in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alkaline Phosphatase
Bone Marrow Cells
Bone Regeneration
Calcification, Physiologic
Cell Adhesion
Cell Differentiation
Cell Proliferation
Ceramics
Glass
Humans
Ions
Manganese
Materials Testing
Microscopy, Fluorescence
Osteoblasts
Osteogenesis
Phase Transition
Silicon Dioxide
Spectroscopy, Fourier Transform Infrared