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Quantification of varying adhesion levels in chondrocytes using the cytodetacher. Ann Biomed Eng 2002 May;30(5):703-12

Date

07/11/2002

Pubmed ID

12108844

DOI

10.1114/1.1484218

Scopus ID

2-s2.0-0035990856   21 Citations

Abstract

The potential to promote cell adhesion must be evaluated in the development of tissue-engineered implants and scaffolds. One measure of cell adherence is the force necessary to detach the cell. The objective of this study was to evaluate the cytodetacher (Athanasiou, K. A., et al. Development of the cytodetachment technique to quantify cellular adhesiveness. Biomaterials 20: 2405-2415, 1999), modified to test cells grown on substrata, as a means of calculating cell adhesion forces. Live and formalin-fixed bovine and rabbit chondrocytes underwent cytodetachment to verify that the cytodetacher provides satisfactory resolution to differentiate between live and fixed cells. Fixed cells had significantly greater mechanical adhesiveness than those prepared live: the values for the fixed rabbit and bovine chondrocytes were 1.01 and 1.56 microN, respectively, versus 0.14 and 0.17 microN for the live cells (p<0.05). The sensitivity of the cytodetacher was also gauged by detaching live rabbit chondrocytes seeded for varying amounts of time (40, 80, and 120 min). For the 40, 80, and 120 min time points the maximum detachment forces were found to be significantly different: 2.87 x 10(-2), 6.75 x 10(-2), and 14.30 x 10(-)2 microN, respectively. This study validates the use of the modified cytodetacher as an effective means of evaluating the strength of adhesion of cells attached to a substratum.

Author List

Hoben G, Huang W, Thoma BS, LeBaron RG, Athanasiou KA

Author

Gwendolyn M B Hoben MD Assistant Professor in the Plastic Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cartilage, Articular
Cattle
Cell Adhesion
Cells, Cultured
Chondrocytes
Cycloheximide
Cytological Techniques
Elasticity
Equipment Design
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Species Specificity
Stress, Mechanical
Transducers
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a