Faculty Collaboration Database

Structural Differences in Meibum From Donors After Hematopoietic Stem Cell Transplantations. Cornea 2019 Sep;38(9):1169-1174

Date

07/02/2019

Pubmed ID

31261179

Pubmed Central ID

PMC6687521

DOI

10.1097/ICO.0000000000001935

Scopus ID

2-s2.0-85070746530 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

PURPOSE: Meibum is considered to be a key component of tears that serve to protect the eye, and conformational changes in meibum have not been studied extensively within the population of patients who had hematopoietic stem cell transplantation (HSCT). The aim of this study was to determine possible lipid conformational changes in the meibum of patients who had HSCT.

METHODS: Participants who had HSCT were randomly sampled for this prospective comparative study. Control participants did not have dry eye or had not undergone allogeneic or autologous stem cell transplantation. Fourier-transform infrared spectroscopy was used to measure meibum phase transition.

RESULTS: Meibum was collected from both eyes of 36 donors without dry eye (Mc) and from 22 patients who had undergone HSCT (MHSCT). There were no significant differences between the phase transition parameters based on gender or race. The following were the significant differences (P < 0.0001) between the parameters for Mc compared with MHSCT : lipid order (% trans) at 33.4°C increased from 40 (1) to 54 (2), cooperativity decreased from 7.9 (0.4) to 5.4 (0.3), the phase transition temperature (C) increased from 30.3 (0.4) to 34.2 (0.9), and the magnitude of the phase transition (cm) increased from 4.0 (0.1) to 4.7 (0.5) (standard error of the mean).

CONCLUSIONS: Conformational and thermodynamic differences were observed between Mc and MHSCT. The changes observed in the lipid conformation of meibum from patients receiving HSCTs suggest that meibum composition changes after stem cell transplantation, and clinicians should consider treating the meibomian glands to improve the ocular surface.

Author List

Ramasubramanian A, Blackburn R, Yeo H, Sledge SM, Gully ZN, Singh S, Mehta S, Mehta A, Yappert MC, Borchman D

Author

Aparna Ramasubramanian MD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Aged
Aged, 80 and over
Case-Control Studies
Female
Hematopoietic Stem Cell Transplantation
Humans
Lipids
Male
Meibomian Glands
Middle Aged
Prospective Studies
Transplantation, Autologous
Young Adult