Faculty Collaboration Database

Subcellular stress response and induction of molecular chaperones and folding proteins after transient global ischemia in rats. Brain Res 2009 Jan 16;1249:9-18

Date

11/11/2008

Pubmed ID

18996359

Pubmed Central ID

PMC2670784

DOI

10.1016/j.brainres.2008.10.032

Scopus ID

2-s2.0-58149168845 (requires institutional sign-in at Scopus site)   67 Citations

Abstract

Brain ischemia induces the toxic accumulation of unfolded proteins in vulnerable neurons. This cellular event can trigger the unfolded protein response (UPR) and activate the expression of a number of genes involved in pro-survival pathways. One of the pro-survival pathways involves the sequestration and elimination of misfolded and aggregated proteins. Recent evidence suggests that the endoplasmic reticulum (ER), mitochondria, and cytoplasm respond individually to the accumulation of unfolded proteins by induction of organelle specific molecular chaperones and folding enzymes. This study utilized a rat model of transient (15 min) global ischemia (2-vessel occlusion) to investigate the regional and temporal induction of some of these key stress proteins after ischemia. Electron microscopy demonstrated that visible protein aggregates accumulated predominately in the cytoplasm. We used in situ hybridization (forebrain structures) and western blot (hippocampus) analysis to measure changes in expression of heat shock protein 70 (HSP70 cytoplasmic), HSP60 (mitochondrial), ER luminal proteins glucose response proteins GRP78 and GRP94, protein disulphide isomerase (PDI), homocysteine-inducible, endoplasmic reticulum stress-inducible protein (HERP), and calnexin. Induction of mRNA for HSP70 occurred earlier (beginning at 30 min) and at a higher level relative to the delayed (4-24 h) and more moderate induction of mRNAs for mitochondrial matrix HSP60 and the ER lumen HERP, GRP78, GRP94, calnexin and PDI. Increases in hippocampal proteins were observed at 4 h (HSP70) and 24 h (HSP60, GRP78, GRP94) after reperfusion. These results demonstrate that after a transient ischemic insult, the subcellular responses to the accumulation of unfolded proteins varies between cellular compartments and are most prevalent in the cytoplasm and, to a lesser degree, in the mitochondrial matrix and ER lumen.

Author List

Truettner JS, Hu K, Liu CL, Dietrich WD, Hu B

Author

Kurt Hu MD Assistant Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Brain
Cell Death
Cerebral Cortex
Chaperonin 60
Cytoplasm
Endoplasmic Reticulum
HSP70 Heat-Shock Proteins
Hippocampus
In Situ Hybridization
Ischemic Attack, Transient
Microscopy, Electron
Molecular Chaperones
Neurons
Protein Folding
RNA, Messenger
Rats
Rats, Wistar
Stress, Physiological
Up-Regulation