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A novel arresting solution for study of postmortem pressure--volume curves of the rat left ventricle. J Surg Res 1998 Dec;80(2):221-8

Date

01/08/1999

Pubmed ID

9878317

DOI

10.1006/jsre.1998.5458

Scopus ID

2-s2.0-0032418363   8 Citations

Abstract

OBJECTIVE: We have found diastolic properties of the rat heart extremely sensitive to the method used to induce arrest. Accordingly, we sought to develop a reliable solution for measuring the LV pressure-volume relationship (LVPVR) in the rat.

MATERIALS AND METHODS: The study had five phases: (i) K120Na100, consisting of KCl (120 mEq/L) in NaCl (100 mEq/L) diluted with distilled water was developed in preliminary experiments. (ii) ACI rats were arrested with 3 cc of 4 degreesC KCl (n = 6) or K120Na100 (n = 6) infused into the aortic root. The LVPVR was expressed as normalized volume (Vn) at standardized pressures. Myocardial water content (%MWC) was determined. (iii) Six hearts were arrested with K120Na100 and LVPVRs observed over 1 h. (iv) Four hearts were instrumented with sonomicrometry crystals to compare in vivo and postmortem pressure diameter data. (v) The relation between body weight and dry heart weight was determined in 48 animals.

RESULTS: In hearts arrested with KCl, mean Vn at pressures of 10, 15, and 20 mmHg (206 +/- 26, 306 +/- 21, and 336 +/- 25 microl, respectively) was significantly reduced vs K120Na100 hearts (345 +/- 11, 407 +/- 12, and 472 +/- 18 microl) (P < 0.05). Mean %MWC changed insignificantly. Vn at 20 mm Hg became significantly smaller vs initial data 60 min after arrest with K120Na100 (P < 0.05, ANOVA). No differences between in vivo and postmortem mean normalized diameter were observed. The correlation coefficient for the relation between body weight and dry heart weight was 0.80. Conclusions. K120Na100 at 4 degreesC reliably preserves LV diastolic properties in the rat heart for 30 min. Normalization of LV volume to body weight is justified.

Author List

Rabkin DG, Jia CX, Cabreriza SE, Hart JP, Starr JP, Spotnitz HM

Author

Joseph Hart MD Associate Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Blood Pressure
Body Weight
Cardioplegic Solutions
Diastole
Evaluation Studies as Topic
Heart
Heart Arrest, Induced
Male
Organ Size
Postmortem Changes
Potassium Chloride
Rats
Rats, Inbred ACI
Sodium Chloride
Ventricular Function, Left