Faculty Collaboration Database

The oxidation of alpha-tocopherol in human low-density lipoprotein by the simultaneous generation of superoxide and nitric oxide. FEBS Lett 1993 Jul 12;326(1-3):199-203

Date

07/12/1993

Pubmed ID

8391994

DOI

10.1016/0014-5793(93)81790-7

Scopus ID

2-s2.0-0027158630 (requires institutional sign-in at Scopus site)   141 Citations

Abstract

Peroxynitrite is the product of the reaction between nitric oxide and superoxide. It is an oxidant which can also decompose to form the hydroxyl radical and nitrogen dioxide. In this report we show that a powerful oxidant with reactivity similar to that of the hydroxyl radical is formed from the generation of superoxide from xanthine oxidase and nitric oxide from S-nitroso-n-acetylpenicillamine (SNAP). Simultaneous generation of these two radicals by either xanthine oxidase/SNAP or the sydnonimine SIN-1 in the presence of low-density lipoprotein (LDL) results in the depletion of alpha-tocopherol and formation of its oxidised product alpha-tocopheroquinone. The mechanism of oxidation required both the formation of nitric oxide and superoxide. In contrast to the promotion of LDL oxidation by transition metals the oxidation of LDL by SIN-1 was not sensitive to the addition of exogenous lipid hydroperoxide.

Author List

Hogg N, Darley-Usmar VM, Wilson MT, Moncada S

Author

Neil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acetaldehyde
Catalase
Free Radicals
Humans
Hydroxides
Hydroxyl Radical
Lipoproteins, LDL
Molsidomine
Nitric Oxide
Nitrogen Dioxide
Oxidation-Reduction
Penicillamine
S-Nitroso-N-Acetylpenicillamine
Superoxide Dismutase
Superoxides
Thiobarbiturates
Vitamin E
Xanthine Oxidase