Validating a low-cost laser speckle contrast imaging system as a quantitative tool for assessing retinal vascular function. Sci Rep 2020 Apr 28;10(1):7177
Date
04/30/2020Pubmed ID
32346043Pubmed Central ID
PMC7188677DOI
10.1038/s41598-020-64204-zScopus ID
2-s2.0-85083998758 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
The ability to monitor progression of retinal vascular diseases like diabetic retinopathy in small animal models is often complicated by their failure to develop the end-stage complications which characterize the human phenotypes in disease. Interestingly, as micro-vascular dysfunction typically precedes the onset of retinal vascular and even some neurodegenerative diseases, the ability to visualize and quantify hemodynamic changes (e.g. decreased flow or occlusion) in retinal vessels may serve as a useful diagnostic indicator of disease progression and as a therapeutic outcome measure in response to treatment. Nevertheless, the ability to precisely and accurately quantify retinal hemodynamics remains an unmet challenge in ophthalmic research. Herein we demonstrate the ability to modify a commercial fundus camera into a low-cost laser speckle contrast imaging (LSCI) system for contrast-free and non-invasive quantification of relative changes to retinal hemodynamics over a wide field-of-view in a rodent model.
Author List
Patel DD, Lipinski DMAuthor
Daniel M. Lipinski PhD Associate Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Flow Velocity
Diabetic Retinopathy
Female
Laser-Doppler Flowmetry
Male
Mice
Microcirculation
Retinal Vessels
