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Coupling of energy intake and energy expenditure across a temperature spectrum: impact of diet-induced obesity in mice. Am J Physiol Endocrinol Metab 2020 09 01;319(3):E472-E484

Date

07/22/2020

Pubmed ID

32691631

Pubmed Central ID

PMC7509245

DOI

10.1152/ajpendo.00041.2020

Scopus ID

2-s2.0-85089821961   1 Citation

Abstract

Obesity and its metabolic sequelae are implicated in dysfunction of the somatosensory, sympathetic, and hypothalamic systems. Because these systems contribute to integrative regulation of energy expenditure (EE) and energy intake (EI) in response to ambient temperature (Ta) changes, we hypothesized that diet-induced obesity (DIO) disrupts Ta-associated EE-EI coupling. C57BL/6N male mice were fed a high-fat diet (HFD; 45% kcal) or low-fat diet (LFD; 10% kcal) for a??9.5 wk; HFD mice were then split into body weight (BWT) quartiles (n = 8 each) to study DIO-low gainers (Q1) versus -high gainers (Q4). EI and indirect calorimetry (IC) were measured over 3 days each at 10A?C, 20A?C, and 30A?C. Responses did not differ between LFD, Q1, and Q4; EI and BWT-adjusted EE increased rapidly when transitioning toward 20A?C and 10A?C. In all groups, EI at 30A?C was not reduced despite lower EE, resulting in positive energy balance and respiratory exchange ratios consistent with increased de novo lipogenesis, energy storage, and relative hyperphagia. We conclude that 1) systems controlling Ta-dependent acute EI/EE coupling remained intact in obese mice and 2) rapid coupling of EI/EE at cooler temperatures is an important adaptation to maintain energy stores and defend body temperature, but less critical at thermoneutrality. A post hoc analysis using digestible EI plus IC-calculated EE suggests that standard IC assumptions for EE calculation require further validation in the setting of DIO. The experimental paradigm provides a platform to query the hypothalamic, somatosensory, and sympathetic mechanisms that drive Ta-associated EI/EE coupling.

Author List

Ono-Moore KD, Rutkowsky JM, Pearson NA, Williams DK, Grobe JL, Tolentino T, Lloyd KCK, Adams SH

Author

Justin L. Grobe PhD Associate Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adipose Tissue, Brown
Animals
Blood Glucose
Body Composition
Body Temperature Regulation
Body Weight
Diet, Fat-Restricted
Diet, High-Fat
Drinking
Energy Intake
Energy Metabolism
Insulin
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity
Temperature