Coupling of energy intake and energy expenditure across a temperature spectrum: impact of diet-induced obesity in mice. Am J Physiol Endocrinol Metab 2020 Sep 01;319(3):E472-E484
Date
07/22/2020Pubmed ID
32691631Pubmed Central ID
PMC7509245DOI
10.1152/ajpendo.00041.2020Scopus ID
2-s2.0-85089821961 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Obesity and its metabolic sequelae are implicated in dysfunction of the somatosensory, sympathetic, and hypothalamic systems. Because these systems contribute to integrative regulation of energy expenditure (EE) and energy intake (EI) in response to ambient temperature (Ta) changes, we hypothesized that diet-induced obesity (DIO) disrupts Ta-associated EE-EI coupling. C57BL/6N male mice were fed a high-fat diet (HFD; 45% kcal) or low-fat diet (LFD; 10% kcal) for ∼9.5 wk; HFD mice were then split into body weight (BWT) quartiles (n = 8 each) to study DIO-low gainers (Q1) versus -high gainers (Q4). EI and indirect calorimetry (IC) were measured over 3 days each at 10°C, 20°C, and 30°C. Responses did not differ between LFD, Q1, and Q4; EI and BWT-adjusted EE increased rapidly when transitioning toward 20°C and 10°C. In all groups, EI at 30°C was not reduced despite lower EE, resulting in positive energy balance and respiratory exchange ratios consistent with increased de novo lipogenesis, energy storage, and relative hyperphagia. We conclude that 1) systems controlling Ta-dependent acute EI/EE coupling remained intact in obese mice and 2) rapid coupling of EI/EE at cooler temperatures is an important adaptation to maintain energy stores and defend body temperature, but less critical at thermoneutrality. A post hoc analysis using digestible EI plus IC-calculated EE suggests that standard IC assumptions for EE calculation require further validation in the setting of DIO. The experimental paradigm provides a platform to query the hypothalamic, somatosensory, and sympathetic mechanisms that drive Ta-associated EI/EE coupling.
Author List
Ono-Moore KD, Rutkowsky JM, Pearson NA, Williams DK, Grobe JL, Tolentino T, Lloyd KCK, Adams SHAuthor
Justin L. Grobe PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adipose Tissue, BrownAnimals
Blood Glucose
Body Composition
Body Temperature Regulation
Body Weight
Diet, Fat-Restricted
Diet, High-Fat
Drinking
Energy Intake
Energy Metabolism
Insulin
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity
Temperature
