Faculty Collaboration Database

Yttrium-90 Portal Vein Radioembolization in Sprague-Dawley Rats: Dose-Dependent Imaging and Pathological Changes in Normal Liver. Cardiovasc Intervent Radiol 2020 Dec;43(12):1925-1935

Date

08/18/2020

Pubmed ID

32803285

Pubmed Central ID

PMC7655630

DOI

10.1007/s00270-020-02614-2

Scopus ID

2-s2.0-85089448590 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

PURPOSE: Portal vein embolization (PVE) is an established neoadjuvant method to induce future liver remnant hypertrophy prior to surgical resection of hepatic tumors. The purpose of our study was to examine the feasibility of PVE with glass ⁹⁰Y microspheres (Y90 PVE) in Sprague-Dawley rats. We tested the hypothesis that increased doses of Y90 PVE would increase target lobe fibrosis and atrophy.

METHODS: Twenty-two rats were assigned to four groups for Y90 PVE to the right median lobe: very high- (273.8 MBq; n = 2), high- (99.9 MBq; n = 10), medium- (48.1 MBq; n = 5), and low-dose (14.8 MBq; n = 5). An untreated control group included seven rats. ⁹⁰Y PET/CT of ⁹⁰Y distributions confirmed lobar targeting. MRI volumes were measured at baseline, 2-, 4-, 8- and 12-weeks. Explanted hepatic lobes were weighed, sectioned, and stained for H&E and immunohistochemistry. Digitized slides allowed quantitative measurements of fibrosis (20 foci/slide).

RESULTS: Ex vivo measurements confirmed 91-97% activity was localized to the target lobe (n = 4). The percent growth of the target lobe relative to baseline was - 5.0% (95% CI - 17.0-6.9%) for high-, medium dose rats compared to + 18.6% (95% CI + 7.6-29.7%) in the low-dose group at 12-weeks (p = 0.0043). Radiation fibrosis increased in a dose-dependent fashion. Fibrotic area/microsphere was 22,893.5, 14,946.2 ± 2253.3, 15,304.5 ± 4716.6, and 5268.8 ± 2297.2 μm² for very high- (n = 1), high- (n = 4), medium- (n = 3), and low-dose groups (n = 5), respectively.

CONCLUSION: Y90 PVE was feasible in the rat model, resulted in target lobe atrophy, and dose-dependent increases in hepatic fibrosis at 12 weeks. The onset of imaging-based volumetric changes was 8-12 weeks.

Author List

Gordon AC, White SB, Gates VL, Li W, Procissi D, Zhang Z, Harris KR, Kim DH, Mouli SK, Omary RA, Salem R, Larson AC, Lewandowski RJ

Author

Sarah B. White MD, MS, FSIR, FCIRSE Associate Dean, Vice Chair, Professor in the Radiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Chemoembolization, Therapeutic
Liver
Liver Neoplasms
Liver Neoplasms, Experimental
Magnetic Resonance Imaging
Male
Microspheres
Neoplasm Staging
Positron Emission Tomography Computed Tomography
Rats
Rats, Sprague-Dawley
Yttrium Radioisotopes