Faculty Collaboration Database

BBB07 contributes to, but is not essential for, Borrelia burgdorferi infection in mice. Microbiology (Reading) 2020 Oct;166(10):988-994

Date

09/17/2020

Pubmed ID

32936070

Pubmed Central ID

PMC7660918

DOI

10.1099/mic.0.000972

Scopus ID

2-s2.0-85094983555 (requires institutional sign-in at Scopus site)   1 Citation

Abstract

Borrelia burgdorferi, a causative agent of Lyme disease, encodes a protein BBB07 on the genomic plasmid cp26. BBB07 was identified as a candidate integrin ligand based on the presence of an RGD tripeptide motif, which is present in a number of mammalian ligands for β₁ and β₃ integrins . Previous work demonstrated that BBB07 in recombinant form binds to β₁ integrins and induces inflammatory responses in synovial cells in culture. Several transposon mutants in bbb07 were attenuated in an in vivo screen of the transposon library in mice. We therefore tested individual transposon mutant clones in single-strain infections in mice and found that they were attenuated in terms of ID₅₀ but did not have significantly reduced tissue burdens in mice. Based on data presented here we conclude that BBB07 is not essential for, but does contribute to, B. burgdorferi infectivity in mice.

Author List

Hahn B, Anderson P, Lu Z, Danner R, Zhou Z, Hyun N, Gao L, Lin T, Norris SJ, Coburn J

Author

Jenifer Coburn PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Load
Bacterial Proteins
Borrelia burgdorferi
Gene Library
Lyme Disease
Mice
Mice, Inbred C3H
Mutation