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BBB07 contributes to, but is not essential for, Borrelia burgdorferi infection in mice. Microbiology (Reading) 2020 Oct;166(10):988-994



Pubmed ID


Pubmed Central ID




Scopus ID

2-s2.0-85094983555 (requires institutional sign-in at Scopus site)


Borrelia burgdorferi, a causative agent of Lyme disease, encodes a protein BBB07 on the genomic plasmid cp26. BBB07 was identified as a candidate integrin ligand based on the presence of an RGD tripeptide motif, which is present in a number of mammalian ligands for β1 and β3 integrins . Previous work demonstrated that BBB07 in recombinant form binds to β1 integrins and induces inflammatory responses in synovial cells in culture. Several transposon mutants in bbb07 were attenuated in an in vivo screen of the transposon library in mice. We therefore tested individual transposon mutant clones in single-strain infections in mice and found that they were attenuated in terms of ID50 but did not have significantly reduced tissue burdens in mice. Based on data presented here we conclude that BBB07 is not essential for, but does contribute to, B. burgdorferi infectivity in mice.

Author List

Hahn B, Anderson P, Lu Z, Danner R, Zhou Z, Hyun N, Gao L, Lin T, Norris SJ, Coburn J


Jenifer Coburn PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Bacterial Load
Bacterial Proteins
Borrelia burgdorferi
Gene Library
Lyme Disease
Mice, Inbred C3H