Community-acquired pneumonia in children: cell-free plasma sequencing for diagnosis and management. Diagn Microbiol Infect Dis 2019 Jun;94(2):188-191
Date
03/02/2019Pubmed ID
30819624Pubmed Central ID
PMC7125591DOI
10.1016/j.diagmicrobio.2018.12.016Scopus ID
2-s2.0-85061958016 (requires institutional sign-in at Scopus site) 47 CitationsAbstract
Community-acquired pneumonia (CAP) is a common cause of pediatric hospital admission. Empiric antibiotic therapy for hospitalized children with serious CAP now targets the most likely pathogen(s), including those that may demonstrate significant antibiotic resistance. Cell-free plasma next-generation sequencing (CFPNGS) was first made available for Pediatric Infectious Diseases physicians in June 1, 2017, to supplement standard-of-care diagnostic techniques. A retrospective chart review was performed for children hospitalized with CAP between June 1, 2017, and January 22, 2018, to evaluate the impact of CFPNGS. We identified 15 hospitalized children with CAP without other underlying medical conditions for whom CFPNGS was performed. CFPNGS identified a pathogen in 13 of 15 (86%) children compared with 47% for those using standard culture and PCR-based methods alone. Changes in antibiotic management were made in 7 of 15 (47%) of children as a result of CFPNGS.
Author List
Farnaes L, Wilke J, Ryan Loker K, Bradley JS, Cannavino CR, Hong DK, Pong A, Foley J, Coufal NGAuthor
Kathleen Ryan Loker MD, MPH Assistant Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Anti-Bacterial AgentsBacteria
Child
Child, Preschool
Community-Acquired Infections
DNA, Bacterial
Disease Management
Female
High-Throughput Nucleotide Sequencing
Humans
Infant
Male
Molecular Diagnostic Techniques
Plasma
Pneumonia, Bacterial
Retrospective Studies
