Faculty Collaboration Database

The nuclear magnetic resonance metabolic profile: Impact of fasting status. Clin Biochem 2021 Jan;87:85-92

Date

11/08/2020

Pubmed ID

33159964

DOI

10.1016/j.clinbiochem.2020.10.014

Scopus ID

2-s2.0-85096544644 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

INTRODUCTION: Measurement of lipoprotein subclass concentration (-c), particle number (-p), and size (-s) by nuclear magnetic resonance (NMR) has gained traction in the clinical laboratory due to associations between smaller lipid particle sizes and atherogenic risk, especially for LDL-p. The standard protocols for lipoprotein measurements by NMR require fasting blood samples; however, patients may not fast properly before sample collection. The study objective was to evaluate the impact of fasting status on the NMR-based lipid profile and to identify key parameters differentiating between fasting and post-meal specimens.

METHODS: Forty-eight self-reported healthy male and female participants were recruited. Blood was collected after a 12 h fast and 4 h after a high fat meal. Samples were analyzed using the AXINON LipoFIT by NMR assay. The measurements included triglyceride, total cholesterol, IDL-c, and LDL, HDL, VLDL concentration, particle number, and size, as well as glucose, and four amino acids (alanine, valine, leucine and isoleucine).

RESULTS: As expected, triglycerides increased after the meal (58%, p < 0.0001). Significant changes were also observed for VLDL, LDL, and HDL parameters, and the branched chain amino acids. The ratio of Valine*VLDL-c/LDL-c or Isoleucine*VLDL-c/LDL-c provided equally effective differentiation of fasting and post-meal samples. The ratio cutoffs (79.1 and 23.6 when calculated using valine and isoleucine, respectively) had sensitivities of 86% and specificities of 93-95%.

CONCLUSIONS: The clinical impact on NMR results from post-meal samples warrants further evaluation. Algorithms to differentiate fasting and post-meal specimens may be useful in identifying suboptimal specimens.

Author List

Smy L, De Biase I, Genzen JR, Yuzyuk T

Author

Laura Smy PhD Assistant Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Algorithms
Cholesterol, LDL
Cholesterol, VLDL
Fasting
Female
Humans
Isoleucine
Magnetic Resonance Spectroscopy
Male
Postprandial Period
Prospective Studies
Risk Factors
Valine