Faculty Collaboration Database

Active transport of 3-O-methyl-glucose by the small intestine in chronically catheterized rats. J Clin Invest 1995 Jun;95(6):2799-805

Date

06/01/1995

Pubmed ID

7769119

Pubmed Central ID

PMC295965

DOI

10.1172/JCI117984

Scopus ID

2-s2.0-0029047852 (requires institutional sign-in at Scopus site)   74 Citations

Abstract

A method is described for determining the fraction of intestinal 3-O-methyl-glucose (3OMG) absorption that occurs by active transport in chronically catheterized rats without the influence of anesthesia or surgical bowel manipulation. That fraction was determined by simultaneously measuring portal venous-aortic blood concentration gradients (delta C) of 3-O-methyl-glucose (3OMG) and L-glucose, metabolically inert analogues of D-glucose. 3OMG is actively and passively absorbed by the same mechanisms as D-glucose, L-glucose is only passively absorbed. The fraction of 3OMG that is actively transported was calculated from the difference between 3OMG and L-glucose absorption, divided by total 3OMG absorption. We found that more than 94% of 3-O-methyl-glucose is absorbed by active transport when luminal concentrations range from 50 to 400 mM. We conclude that in unrestrained, unanesthetized chronically catheterized rats, most 3OMG is actively absorbed by the intestine even at high luminal concentrations.

Author List

Uhing MR, Kimura RE

Author

Michael R. Uhing MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

3-O-Methylglucose
Animals
Biological Transport, Active
Catheterization
Intestinal Absorption
Intestine, Small
Male
Methylglucosides
Phlorhizin
Rats
Rats, Sprague-Dawley