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The effect of surgical bowel manipulation and anesthesia on intestinal glucose absorption in rats. J Clin Invest 1995 Jun;95(6):2790-8

Date

06/01/1995

Pubmed ID

7769118

Pubmed Central ID

PMC295964

DOI

10.1172/JCI117983

Scopus ID

2-s2.0-0029078054 (requires institutional sign-in at Scopus site)   78 Citations

Abstract

The effects of surgical bowel manipulation and anesthesia on intestinal glucose absorption were determined in chronically catheterized rats. Total and passive rates of glucose absorption were measured using 3-O-methyl-glucose (3OMG) and L-glucose, metabolically inert analogues of D-glucose. The rates of 3OMG absorption immediately postoperative and 4 h later were 86 and 62% less than the absorption rate 6 d postoperative. The absorption rates of 3OMG 1 and 2 d postoperative were not different from 6 d postoperative. Absorption of L-glucose was not altered by bowel manipulation and anesthesia. Even after correction for the increased resistance of the unstirred water layer (UWL) after bowel manipulation, the rates of total and active intestinal glucose absorption immediately postoperative were only 11 and 15% of predicted rates of absorption. In chronically catheterized rats, > 75% of luminal 3OMG at a concentration of 400 mM was absorbed by active transport. The Km and Vmax of 3OMG active transport corrected for the resistance of the UWL were 11.3 mM and 15.6 mumoles/min, respectively. We conclude that measurements of intestinal glucose absorption performed within 24 h of surgical bowel manipulation greatly underestimate active absorption even if corrections are made to account for the increased resistance of the UWL.

Author List

Uhing MR, Kimura RE

Author

Michael R. Uhing MD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

3-O-Methylglucose
Anesthesia
Animals
Biological Transport, Active
Catheterization
Glucose
Intestinal Absorption
Intestines
Laparotomy
Male
Methylglucosides
Perfusion
Polyethylene Glycols
Rats
Rats, Sprague-Dawley