Neutrophil-Derived Myeloperoxidase Facilitates Both the Induction and Elicitation Phases of Contact Hypersensitivity. Front Immunol 2020;11:608871
Date
02/12/2021Pubmed ID
33569056Pubmed Central ID
PMC7868335DOI
10.3389/fimmu.2020.608871Scopus ID
2-s2.0-85100694615 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
BACKGROUND: Allergic contact dermatitis (ACD) is a common skin disorder affecting an estimated 15-20% of the general population. The mouse model of ACD is contact hypersensitivity (CHS), which consists of two phases: induction and elicitation. Although neutrophils are required for both CHS disease phases their mechanisms of action are poorly understood. Neutrophils release myeloperoxidase (MPO) that through oxidation of biomolecules leads to cellular damage.
OBJECTIVES: This study investigated mechanisms whereby MPO contributes to CHS pathogenesis.
METHODS: CHS was induced in mice using oxazolone (OX) as the initiating hapten applied to the skin. After 7 days, CHS was elicited by application of OX to the ear and disease severity was measured by ear thickness and vascular permeability in the ear. The role of MPO in the two phases of CHS was determined utilizing MPO-deficient mice and a specific MPO inhibitor.
RESULTS: During the CHS induction phase MPO-deficiency lead to a reduction in IL-1β production in the skin and a subsequent reduction in migratory dendritic cells (DC) and effector T cells in the draining lymph node. During the elicitation phase, inhibition of MPO significantly reduced both ear swelling and vascular permeability.
CONCLUSION: MPO plays dual roles in CHS pathogenesis. In the initiation phase MPO promotes IL-1β production in the skin and activation of migratory DC that promote effector T cell priming. In the elicitation phase MPO drives vascular permeability contributing to inflammation. These results indicate that MPO it could be a potential therapeutic target for the treatment of ACD in humans.
Author List
Strzepa A, Gurski CJ, Dittel LJ, Szczepanik M, Pritchard KA Jr, Dittel BNAuthor
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Movement
Dendritic Cells
Dermatitis, Allergic Contact
Dermatitis, Contact
Haptens
Inflammation
Interleukin-1beta
Lymph Nodes
Mice
Mice, Inbred C57BL
Neutrophils
Oxazolone
Peroxidase
Skin
T-Lymphocytes
