Faculty Collaboration Database

High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging. Proc Natl Acad Sci U S A 2020 Dec 08;117(49):31448-31458

Date

11/25/2020

Pubmed ID

33229571

Pubmed Central ID

PMC7733854

DOI

10.1073/pnas.2014389117

Scopus ID

2-s2.0-85097581685 (requires institutional sign-in at Scopus site)   32 Citations

Abstract

Adult neural stem cells (NSC) serve as a reservoir for brain plasticity and origin for certain gliomas. Lineage tracing and genomic approaches have portrayed complex underlying heterogeneity within the major anatomical location for NSC, the subventricular zone (SVZ). To gain a comprehensive profile of NSC heterogeneity, we utilized a well-validated stem/progenitor-specific reporter transgene in concert with single-cell RNA sequencing to achieve unbiased analysis of SVZ cells from infancy to advanced age. The magnitude and high specificity of the resulting transcriptional datasets allow precise identification of the varied cell types embedded in the SVZ including specialized parenchymal cells (neurons, glia, microglia) and noncentral nervous system cells (endothelial, immune). Initial mining of the data delineates four quiescent NSC and three progenitor-cell subpopulations formed in a linear progression. Further evidence indicates that distinct stem and progenitor populations reside in different regions of the SVZ. As stem/progenitor populations progress from neonatal to advanced age, they acquire a deficiency in transition from quiescence to proliferation. Further data mining identifies stage-specific biological processes, transcription factor networks, and cell-surface markers for investigation of cellular identities, lineage relationships, and key regulatory pathways in adult NSC maintenance and neurogenesis.

Author List

Xie XP, Laks DR, Sun D, Poran A, Laughney AM, Wang Z, Sam J, Belenguer G, Fariñas I, Elemento O, Zhou X, Parada LF

Author

Daochun Sun PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult Stem Cells
Aging
Animals
Biomarkers
Cell Lineage
Green Fluorescent Proteins
Humans
Lateral Ventricles
Mice
Neural Stem Cells
Stem Cell Niche
Transcriptome
Transgenes