Dynamic control of extracellular environment in in vitro neural recording systems. IEEE Trans Neural Syst Rehabil Eng 2005 Jun;13(2):207-12
Date
07/12/2005Pubmed ID
16003901DOI
10.1109/TNSRE.2005.848685Scopus ID
2-s2.0-22544485390 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
A technique is presented for rapid fabrication of microfluidic channels on top of multichannel in vitro neural recording electrode arrays. The channels allow dynamic control of both stable and transient flow patterns over localized areas of the array, over biologically relevant timescales. A cellular model consisting of thermally sensitive dorsal root ganglion neurons was integrated into the devices. The device was used to demonstrate precise control of the extracellular microenvironment of individual cells on the array. Since the methods presented here are not specific to a particular cell type or neural recording system, the technique is amenable to a wide range of applications within the neuroscience field.
Author List
Pearce TM, Williams JJ, Kruzel SP, Gidden MJ, Williams JCAuthor
Jordan J. Williams MD, PhD Assistant Professor in the Biomedical Engineering department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AnimalsCell Adhesion
Cell Culture Techniques
Cell Line
Cells, Cultured
Equipment Design
Equipment Failure Analysis
Extracellular Fluid
Mice
Microelectrodes
Microfluidic Analytical Techniques
Neurons
Systems Integration
