Red light stimulates vasodilation through extracellular vesicle trafficking. J Photochem Photobiol B 2021 Jul;220:112212
Date
05/29/2021Pubmed ID
34049180Pubmed Central ID
PMC8240139DOI
10.1016/j.jphotobiol.2021.112212Scopus ID
2-s2.0-85107807187 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Red light (670 nm) promotes ex vivo dilation of blood vessels in a nitric oxide (NO) dependent, but eNOS independent manner by secreting a quasi-stable and transferable vasoactive substance with the characteristics of S-nitrosothiols (RSNO) from the endothelium. In the present work we establish that 670 nm light mediated vasodilation occurs in vivo and is physiologically stable. Light exposure depletes intracellular S-nitroso protein while concomitantly increasing extracellular RNSO, suggesting vesicular pathways are involved. Furthermore, we demonstrate this RSNO vasodilator is embedded in extracellular vesicles (EV). The action of red light on vesicular trafficking appears to increase expression of endosome associated membrane protein CD63 in bovine aortic endothelial cells, enhance endosome localization in the endothelium, and induce exit of RSNO containing EVs from murine facialis arteries. We suggest a mechanism by which the concerted actions of 670 nm light initiate formation of RSNO containing EVs which exit the endothelium and trigger relaxation of smooth muscle cells.
Author List
Weihrauch D, Keszler A, Lindemer B, Krolikowski J, Lohr NLAuthors
Agnes Keszler PhD Research Scientist I in the Biophysics department at Medical College of WisconsinDorothee Weihrauch DVM, PhD Research Scientist II in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCattle
Cells, Cultured
Endothelium, Vascular
Extracellular Vesicles
Light
Mice
Mice, Inbred C57BL
Nitroso Compounds
Sulfhydryl Compounds
Vasodilation
