The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody. Gene Expr Patterns 2018 Jan;27:114-121
Date
12/12/2017Pubmed ID
29225067Pubmed Central ID
PMC5835195DOI
10.1016/j.gep.2017.11.006Scopus ID
2-s2.0-85037691726 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
The Atoh7 transcription factor catalyzes the rate-limiting step in the specification of retinal ganglion cells (RGCs). As a tool to study vertebrate retinal development, we validate an antibody that recognizes human and mouse Atoh7 polypeptide, using informative knockout and transgenic mouse tissues and overexpression experiments. The transient features of Atoh7 protein expression during retinal neurogenesis match the expected pattern at the tissue and cellular level. Further, we compare endogenous Atoh7 to established RGC markers, reporter mouse lines and cell cycle markers, demonstrating the utility of the antibody to investigate molecular mechanisms of retinal histogenesis.
Author List
Miesfeld JB, Glaser T, Brown NLAuthor
Joel Bryan Miesfeld PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Antibodies, Monoclonal
Basic Helix-Loop-Helix Transcription Factors
Cells, Cultured
Embryo, Mammalian
Gene Expression Regulation, Developmental
HEK293 Cells
Humans
Mice
Mice, Transgenic
Nerve Tissue Proteins
Neurogenesis
Retina
Retinal Ganglion Cells
Sequence Homology
