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[Expression of M3 receptor gene in peripheral blood lymphocytes of workers exposed to dimethoate]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2004 Feb;22(1):3-6

Date

03/23/2004

Pubmed ID

15033005

Scopus ID

2-s2.0-4243190110   1 Citation

Abstract

OBJECTIVE: To study the expression of muscarinic receptor M(3) gene in peripheral blood lymphocytes of workers exposed to organophosphorus pesticides (OPPs) and to explore its role in the adverse effects of OPPs.

METHODS: The lymphocytes of peripheral blood from 33 workers exposed to dimethoate and 15 control people were isolated and treated with saline and dimethoate respectively in vitro. RT-PCR technique was used to determine M(3) gene expression. Basal and inducible gene expression levels were measured.

RESULTS: There was no significant difference in basal gene expression level between exposed group and control group, while the inducible gene expression level was significantly higher in exposure group (1.92 +/- 1.07) than in control group (1.22 +/- 0.19) and basal level (1.49 +/- 0.45, P < 0.05). No differences in basal and inducible gene expression level were found between male and female people in both exposed and control group. The level of inducible M(3) gene expression increased with the increase in length of exposure time [< 5 a: (1.69 +/- 0.95), 5 - 25 a: (1.91 +/- 1.03), > 25 a: (2.09 +/- 1.25), the latter was significantly different from that of < 5 a (P < 0.05)].

CONCLUSION: After long-term exposure to OPPs, the basal M(3) receptor gene expression level in the exposed workers did not show any difference from the control group, but the inducible gene expression level (treated with dimethoate in vitro) was increased and related to the extent of exposure to dimethoate.

Author List

Sun YG, Zhou ZJ, Zhang XM, Gu XA, Jin TY

Author

Yunguang Sun MD, PhD Assistant Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Dimethoate
Female
Gene Expression
Humans
Insecticides
Lymphocytes
Male
Occupational Exposure
RNA, Messenger
Receptor, Muscarinic M3
Reverse Transcriptase Polymerase Chain Reaction