Neuroaminidase reduces interictal spikes in a rat temporal lobe epilepsy model. Epilepsia 2011 Mar;52(3):e12-5
Date
03/04/2011Pubmed ID
21366554Pubmed Central ID
PMC3265164DOI
10.1111/j.1528-1167.2011.02988.xScopus ID
2-s2.0-79952541120 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Interictal spikes have been implicated in epileptogenesis and cognitive dysfunction in epilepsy. Unfortunately, antiepileptic drugs have shown poor efficacy in suppressing interictal discharges; novel therapies are needed. Surface charge on neuronal membranes provides a novel target for abolishing interictal spikes. This property can be modulated through the use of neuraminidase, an enzyme that decreases the amount of negatively charged sialic acid. In the present report we determined whether applying neuraminidase to brains of rats with a history of status epilepticus would reduce number of interictal discharges. Following pilocarpine-induced status epilepticus, rats received intrahippocampal injections of neuraminidase, which significantly decreased the number of interictal spikes recorded in the CA1 region. This study provides evidence that sialic acid degradation can reduce the number of interictal spikes. Furthermore, the results suggest that modifying surface charge created by negatively charged sialic acid may provide new opportunities for reducing aberrant epileptiform events in epilepsy.
Author List
Isaev D, Zhao Q, Kleen JK, Lenck-Santini PP, Adstamongkonkul D, Isaeva E, Holmes GLAuthor
Olena Isaeva PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnticonvulsants
Convulsants
Electroencephalography
Epilepsy, Temporal Lobe
Evoked Potentials
Gliosis
Hippocampus
Injections
Injections, Intraperitoneal
Lithium Chloride
Male
N-Acetylneuraminic Acid
Neuraminidase
Pilocarpine
Rats
Rats, Sprague-Dawley
Signal Processing, Computer-Assisted
Status Epilepticus
