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Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes. Methods Mol Biol 2021;2158:125-139

Date

08/29/2020

Pubmed ID

32857370

Pubmed Central ID

PMC8221246

DOI

10.1007/978-1-0716-0668-1_10

Scopus ID

2-s2.0-85090177829 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

Failure to regenerate myocardium after injury is a major cause of mortality and morbidity in humans. Direct differentiation of human induced pluripotent stem cells (iPSCs) into cardiomyocytes provides an invaluable resource to pursue cardiac regeneration based on cellular transplantation. Beyond the potential for clinical therapies, iPSC technology also enables the generation of cardiomyocytes to recapitulate patient-specific phenotypes, thus presenting a powerful in vitro cell-based model to understand disease pathology and guide precision medicine. Here, we describe protocols for reprogramming of human dermal fibroblasts and blood cells into iPSCs using the non-integrative Sendai virus system and for the monolayer differentiation of iPSCs to cardiomyocytes using chemically defined media.

Author List

Han L, Mich-Basso J, Kühn B

Author

Lu Han PhD Assistant Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cell Differentiation
Cellular Reprogramming
Dermis
Fibroblasts
Humans
Induced Pluripotent Stem Cells
Myocytes, Cardiac
Regeneration