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Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization. RNA 1995 Dec;1(10):1071-8

Date

12/01/1995

Pubmed ID

8595562

Pubmed Central ID

PMC1369333

Scopus ID

2-s2.0-0029448034 (requires institutional sign-in at Scopus site)   75 Citations

Abstract

This work introduces the first use of fluorescent in situ hybridization (FISH) to detect the distribution of specific transcripts in Saccharomyces cerevisiae. We have applied this technique to analysis of reporter transcripts from a single, integrated copy, or multicopy plasmids. We have evaluated the effect of splice site deletions or the presence or absence of a terminator/cleavage site and demonstrated that both splicing and polyadenylation affect the export of these transcripts from the nucleus to the cytoplasm. Moreover, we show that the exported pre-mRNAs are substrates for nonsense codon-mediated decay through the UPF1 pathway. The work presented here demonstrates that the spatial distribution of transcripts will also be an important component of yeast RNA metabolism.

Author List

Long RM, Elliott DJ, Stutz F, Rosbash M, Singer RH

Author

Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Base Sequence
Consensus Sequence
Genes, Reporter
In Situ Hybridization, Fluorescence
Lac Operon
Molecular Sequence Data
Plasmids
RNA Polymerase II
RNA Precursors
RNA Processing, Post-Transcriptional
RNA, Fungal
RNA, Messenger
Saccharomyces cerevisiae
Sequence Deletion
Transcription, Genetic