Monitoring pH of otitis media effusion in chinchillas using fluorescence spectroscopy. IEEE Trans Biomed Eng 1995 Oct;42(10):1027-32
Date
10/01/1995Pubmed ID
8582720DOI
10.1109/10.464377Scopus ID
2-s2.0-0029380427 (requires institutional sign-in at Scopus site)Abstract
We have developed a fiber optic fluorometer to measure fluorescent signal intensities across an epithelium barrier. As a medically relevant example, we have measured the pH of the effusion formed during Hemophilus influenzae induced otitis media infection in the chinchilla, the classical animal model for human middle ear disease. Because the choice of antibiotic used in clinical therapy is dependent on the pH of the effusion, a noninvasive method of measuring pH is highly desirable. Using the fluorescent pH probe carboxy-seminapthorhodafluor, we were able to detect pH changes of 0.15 units in the pH range around 7.0. The development and resolution of the otitis media was followed with magnetic resonance imaging to confirm the presence of the effusion formed during the infection.
Author List
Magin RL, Oh DK, Zhang A, Webb AG, Thulin JDAuthor
Joseph Thulin DVM Assistant Provost, Professor in the Research Office department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBenzopyrans
Chinchilla
Coloring Agents
Disease Models, Animal
Ear, Middle
Female
Fiber Optic Technology
Haemophilus Infections
Haemophilus influenzae
Hydrogen-Ion Concentration
Indicators and Reagents
Magnetic Resonance Imaging
Male
Monitoring, Physiologic
Naphthols
Optical Fibers
Otitis Media with Effusion
Rhodamines
Spectrometry, Fluorescence
