Lipidomics dataset of Danio rerio optic nerve regeneration model. Data Brief 2021 Aug;37:107260
Date
08/12/2021Pubmed ID
34377754Pubmed Central ID
PMC8327136DOI
10.1016/j.dib.2021.107260Scopus ID
2-s2.0-85110586220 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
The right optic nerve of adult, 6 month to 1 year old, female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured left optic nerves were also collected. The tissue was dissected from euthanized fish and frozen on dry ice. Samples were pooled for each category (female crush, female control, male crush, male control) n = 24 to obtain sufficient tissue for analysis. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [1] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. This data is available at Metabolomics Workbench, Study ID ST001725.
