Hydroxyurea-induced global transcriptional suppression in mouse ES cells. Carcinogenesis 2010 Sep;31(9):1661-8
Date
06/02/2010Pubmed ID
20513671DOI
10.1093/carcin/bgq106Scopus ID
2-s2.0-77956283059 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Hydroxyurea (HU), as a therapeutic medicine, has been extensively used clinically. To further survey molecular mechanisms of HU treatment, we analyzed global transcriptomic alteration of mouse ES cells in response to the treatment using high-throughput sequencing. We show that the global transcriptional activity is significantly suppressed as cells are exposed to HU treatment and alters multiple key cellular pathways, including cell cycle, apoptosis and DNAs. HU treatment also alters alternative splicing mechanisms and suppresses non-coding RNA expression. Our result provides novel clues for the understanding of how cells respond to HU and further suggests that high-throughput sequencing technology provides a powerful tool to study mechanisms of clinical drugs at the cellular level.
Author List
Cui P, Lin Q, Xin C, Han L, An L, Wang Y, Hu Z, Ding F, Zhang L, Hu S, Hang H, Yu JAuthor
Lu Han PhD Assistant Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsApoptosis
Biomarkers, Tumor
Blotting, Western
Cell Cycle
Cell Proliferation
Cells, Cultured
Embryonic Stem Cells
Gene Expression Profiling
Hydroxyurea
Mice
Nucleic Acid Synthesis Inhibitors
Oligonucleotide Array Sequence Analysis
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic
