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Pancreatic antioxidant enzyme activity in normoglycemic diabetic prone BB rats. Pancreas 1995 Jan;10(1):53-8

Date

01/01/1995

Pubmed ID

7899460

DOI

10.1097/00006676-199501000-00007

Scopus ID

2-s2.0-0028836705   14 Citations

Abstract

Free radicals have been implicated in beta-cell destruction. pancreatic antioxidant enzymatic defenses provide significant endogenous protection from these highly cytotoxic effector molecules. In our colony of BB rats, the reliability of peripheral blood lymphocyte count (PBLC) as a predictor of diabetes onset was assessed in 99 male and 110 female BB rats. In rats with a PBLC < 4,200 mm3, 90% of males and 86% of females developed diabetes by 120 days of age and are designated "lymphopenic" or BBL. Rats with a PBLC > 4,200 mm3 have a much lower incidence of insulin-dependent diabetes mellitus (IDDM) and are designated "nonlymphopenic" or BBNL. In separate cohorts of normoglycemic (prediabetic) BBL rats (high risk for developing diabetes) and BBNL rats (low risk for developing diabetes), the activities of pancreatic cytosolic antioxidant enzymes, copper-zinc superoxide dismutase (CuZnSOD), catalase (CAT), and glutathione peroxidase (GPX) were determined. Alterations in CuZnSOD and CAT were noted in BBL males only as compared to BBNL males and BBL and BBNL females. CuZnSOD and CAT activities were significantly lower in BBL males. GPX showed a similar trend. The changes in pancreatic antioxidant enzymes precede overt hyperglycemia in male rats at risk for development of diabetes and may result in increased beta-cell vulnerability to oxygen-derived free radical destruction.

Author List

Roza AM, Pieper GM, Johnson CP, Adams MB

Authors

Christopher P. Johnson MD Professor in the Surgery department at Medical College of Wisconsin
Allan M. Roza MD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Antioxidants
Blood Glucose
Catalase
Cytosol
Diabetes Mellitus, Type 1
Disease Susceptibility
Female
Free Radicals
Glutathione Peroxidase
Male
Pancreas
Rats
Rats, Inbred BB
Reference Values
Superoxide Dismutase
jenkins-FCD Prod-478 d1509cf07a111124a2d122fd3df854cc0b993c00