An angiographic method for in vivo study of arteries of the circle of Willis in small animals. Am J Physiol 1992 Nov;263(5 Pt 2):H1616-22
Date
11/01/1992Pubmed ID
1443212DOI
10.1152/ajpheart.1992.263.5.H1616Scopus ID
2-s2.0-0026464492 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
An X-ray imaging technique designed to allow sequential diameter measurements of the cerebral vessels in intact, anesthetized small animals under relatively physiological conditions is described. The ferret and the rabbit were chosen as potentially useful animal models for studying the cerebrovascular system because of the advantageous anatomic characteristics of these relatively small species. A commercially available and relatively inexpensive X-ray imaging system with a small focal spot provides good spatial resolution. An external carotid perfusion loop allows for 1) the introduction of low-osmolality contrast medium without changing perfusion pressure or flow and 2) measurement of internal carotid and circle of Willis pressures at the same time that the vessel images are obtained. In the present study, detection of small changes in the diameters of the small vessels is facilitated by an algorithm utilizing the X-ray absorption by the entire vessel cross section. This avoids some of the problems of edge detection for small cylindrical vessels wherein the contrast is less than optimal and diminishes as the vessel perimeter is approached.
Author List
Harder DR, Schulte ML, Clough AV, Dawson CAAuthor
Anne Clough PhD Professor in the Mathematics, Statistics, and Computer Science department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AnimalsCarotid Arteries
Cerebral Angiography
Cerebral Arteries
Circle of Willis
Equipment and Supplies
Ferrets
Mathematics
Papaverine
Rabbits
Time Factors
Videotape Recording