Faculty Collaboration Database

VEGF is critical for spontaneous differentiation of stem cells into cardiomyocytes. Biochem Biophys Res Commun 2007 Mar 23;354(4):999-1003

Date

02/06/2007

Pubmed ID

17276389

DOI

10.1016/j.bbrc.2007.01.095

Scopus ID

2-s2.0-33846829796 (requires institutional sign-in at Scopus site)   121 Citations

Abstract

Cardiomyocyte regeneration is limited in adult life and is not sufficient to compensate for cell loss with myocardial infarction. Hence, the identification of a useful source of cardiomyocyte progenitors is of great interest for possible use in regenerative therapy. In this study, we isolated stem cells derived from human subcutaneous adipose tissue. The expression of Nkx2.5 and GATA-4 can be observed by PCR directly after extraction and during cultivation in some of these cells. Cardiac Troponin T and myosin light chain-2v become positive after 12 days of cultivation. To define respective factors responsible for spontaneous differentiation, we measured VEGF level in ADSC conditioned medium. Our data showed that ADSC secrete significant amount of VEGF (283.5pg per microgram DNA) and that anti-VEGF receptor antibodies blocked the cardiac differentiation. In conclusion, we demonstrated the spontaneous differentiation of human subcutaneous adipose-derived stem cells into a cardiomyocyte phenotype under standard culturing conditions.

Author List

Song YH, Gehmert S, Sadat S, Pinkernell K, Bai X, Matthias N, Alt E

Author

Xiaowen Bai PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cardiac Myosins
Cell Differentiation
Cells, Cultured
GATA4 Transcription Factor
Homeodomain Proteins
Humans
Myocytes, Cardiac
Myosin Light Chains
Rats
Stem Cells
Subcutaneous Fat
Transcription Factors
Troponin T
Vascular Endothelial Growth Factor A