Faculty Collaboration Database

Alteration in levels of immunoreactive epidermal growth factor in the gastrointestinal mucosa of Fischer rats fed a diet containing 10% wheat bran. J Nutr 1991 Jun;121(6):800-5

Date

06/01/1991

Pubmed ID

2033465

DOI

10.1093/jn/121.6.800

Scopus ID

2-s2.0-0025864143 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

This study evaluates the effect of dietary wheat bran on the levels of immunoreactive epidermal growth factor (EGF) in the gastrointestinal mucosa of Fischer 344 rats. Male rats were fed either a fiber-free diet or a diet containing 10% wheat bran (nine animals per group) for a period of 5 wk. The gastrointestinal tract of each animal was divided into four segments: proximal, middle and distal small intestine, and colon. Mucosa was removed by scraping, EGF was extracted by homogenization and the extracts were analyzed for immunoreactive rat EGF using a homologous RIA. Levels of immunoreactive EGF in all regions of the small intestine of Fischer rats were comparable to our previous measurements in Sprague-Dawley rats, and these levels were unaffected by diet. In contrast, the EGF levels in the colon of the Fischer rats were approximately fivefold greater than those of the Sprague-Dawley rats. These higher levels of immunoreactive EGF in the colon decreased 63% with the addition of 10% wheat bran to the diet (P less than 0.02). These results represent the first demonstration of dietary fiber modulating the content of EGF in the gastrointestinal tract.

Author List

Schaudies RP, Satchithanandam S, Calvert RJ

Author

Lu Han PhD Assistant Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Diet, Reducing
Eating
Epidermal Growth Factor
Intestinal Mucosa
Male
Proteins
Radioimmunoassay
Rats
Rats, Inbred F344
Rats, Inbred Strains
Triticum
Weight Gain