Physico-chemical modeling of the role of free radicals in photodynamic therapy. IV. Quantitative aspects of photodynamic effects on free radicals generated in cell cultures. Biochem Biophys Res Commun 1999 Feb 16;255(2):360-6
Date
03/02/1999Pubmed ID
10049713DOI
10.1006/bbrc.1999.0193Scopus ID
2-s2.0-0038441176 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Production and the mechanism of the interactions of free radicals generated by stimulated macrophages in the presence of luminol and a free radical inhibitor was investigated to determine the possibility of using luminol-dependent chemiluminescence for studying photodynamic effects in biology. Earlier measurements have been revisited and additional experiments performed indicating that oxidation products of luminol neither inhibit the in vitro formation of radicals nor quench CL. Simulation based on the mechanism suggested revealed that the likely value for the rate constant of the primary step between luminol and superoxide anion radicals producing luminol radicals is 5x10(2)-1x10(3) M-1s-1. It has been established that the ratio of the concentration of radicals generated by the biological system to that formed by oxidation of luminol exceeds 10(3); that is, the contribution of the latter is negligible and the system is appropriate to measure quantitatively the effect of excited photosensitizers on free radicals.
Author List
Németh A, Jakus J, Kriska T, Keszler A, Vanyur R, Gál DAuthors
Agnes Keszler PhD Research Scientist I in the Biophysics department at Medical College of WisconsinTamas Kriska PhD Research Scientist I in the Pharmacology and Toxicology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCells, Cultured
Free Radicals
Luminescent Measurements
Luminol
Macrophages, Peritoneal
Male
Mice
Mice, Inbred DBA
Models, Biological
Models, Chemical
Photochemotherapy
Respiratory Burst
Tetradecanoylphorbol Acetate
Zymosan
