Faculty Collaboration Database

Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex. Cell Immunol 1993 Feb;146(2):270-83

Date

02/01/1993

Pubmed ID

8174170

DOI

10.1006/cimm.1993.1026

Scopus ID

2-s2.0-0027408731 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

PNK-E and G7 mAbs regulate porcine NK and ADCC activities by binding to distinct NK function-associated trigger molecules on porcine NK cells. Previous work demonstrates that PNK-E mAb binds to a 205-kDa tetrameric molecule composed of two 47-kDa peptides and two 50-kDa peptides and G7 mAb binds to a distinct 40-kDa heterodispersed monomeric peptide on porcine NK cells. The data presented herein demonstrate that all PNK-E+ PBLs are G7+ and all G7+ PBLs are PNK-E+ indicating that the PNK-E and G7 molecules are coexpressed by porcine NK cells. Bound G7 mAb blocks subsequent binding of PNK-E mAb but not the converse. Bound F(ab')2 G7 mAb abrogates the ability of whole PNK-E mAb to enhance NK activity but bound F(ab')2 PNK-E mAb has no affect on G7 mAb enhancement of NK activity. PNK-E mAb enhanced NK activity is inhibited by binding of F(ab')2 G7 mAb even though whole PNK-E mAb remains bound. However, bound F(ab')2 PNK-E mAb has no affect on G7 mAb-enhanced NK activity. When PNK-E and G7 mAbs were tested alone and together in NK assays, comparable levels of enhancement were observed. PNK-E and G7 hybridomas express surface mAb through which NK cells bind and specifically lyse these hybridomas. Lysis of PNK-E and G7 hybridomas is inhibited by pretreatment of PBLs with F(ab')2 G7 mAb. These data indicate a physical association between the PNK-E and G7 molecules on NK cells and suggest that the G7 molecule is external to the PNK-E molecule.

Author List

Wierda WG, Johnson BD, Dato ME, Kim YB

Author

Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Cell Separation
Centrifugation, Density Gradient
Cytotoxicity, Immunologic
Female
Flow Cytometry
Humans
Immunoglobulin Fab Fragments
Killer Cells, Natural
Male
Mice
Mice, Inbred BALB C
Receptors, Immunologic
Swine
Swine, Miniature
Tumor Cells, Cultured