Faculty Collaboration Database

In vivo experimental studies on the role of free radicals in photodynamic therapy. III. Photodynamic effect on free radicals generated in cell cultures. Biochem Biophys Res Commun 1997 Apr 07;233(1):173-6

Date

04/07/1997

Pubmed ID

9144417

DOI

10.1006/bbrc.1997.6416

Scopus ID

2-s2.0-0031557656 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

The effect of excited Photofrin II molecules on the zymosan stimulated and luminol dependent chemiluminescence (CL) of macrophages, known as respiratory burst, has been studied. Excitation was carried out by light irradiation in the vicinity of the maximal CL by varying light doses. In separate experiments potassium salt of 3,5-di-tert-butyl-4-hydroxyphenyl propionic acid radical inhibitor was added to the system and its consumption measured spectroscopically. Based on the experimental results the decrease in the steady state radical concentrations under the influence of radical inhibitor and of triplet sensitizer generated in situ has been calculated. It has been established that the corresponding photodynamic effect is an inhibition-like process proceeding by triplet-doublet interactions.

Author List

Gál D, Kriska T, Maltseva E

Author

Tamas Kriska PhD Research Scientist I in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cells, Cultured
Free Radical Scavengers
Free Radicals
Luminescent Measurements
Macrophages
Male
Mice
Mice, Inbred DBA
Photochemotherapy