Faculty Collaboration Database

[Demonstration of an intact complex of epidermal growth factor and its receptor in the endosomes of A-431 cells]. Tsitologiia 1988 Mar;30(3):291-8

Date

03/01/1988

Pubmed ID

3261906

Scopus ID

2-s2.0-0023880842 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

The compartmentalization of the epidermal growth factor (EGF) receptors in A-431 cells was studied using centrifugation of the microsomal fraction of these cells in continuous Percoll gradient. The existence of an intact (non-degraded) EGF receptor in plasma membrane and endosome fraction was demonstrated by electrophoretic analysis of in vitro phosphorylated Percoll fractions. No phosphorylated receptor was revealed in lysosomal fraction by this method. The existence of non dissociated EGF-receptor complexes in intracellular compartments 30 minutes after the start of internalization was proven using a synthesized photoreactive labeled EGF derivative (125I-EGF-SANAH). The removing of pH gradient in organellar membranes by 10 mkM of monensin did not affect dissociation from its receptor. The data obtained proved the existence of non-dissociated and non-degraded EGF-receptor complexes in the endosomal compartment of A-431 cells.

Author List

Kornilova ES, Bobrova IF, Nesterov AM, Nikol'skiĭ NN, Sorokin AB

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Carcinoma, Squamous Cell
Cell Compartmentation
Cell Fractionation
Cell Line
Electrophoresis, Polyacrylamide Gel
Epidermal Growth Factor
ErbB Receptors
Humans
Microscopy, Electron
Organoids
Phosphorylation
Tumor Cells, Cultured