Faculty Collaboration Database

[Amine inhibition of serum-induced DNA synthesis in 3T3 and 3T6 cell cultures]. Tsitologiia 1985 Apr;27(4):451-7

Date

04/01/1985

Pubmed ID

4002341

Scopus ID

2-s2.0-0022053888 (requires institutional sign-in at Scopus site)

Abstract

Several amines were shown to inhibit growth stimulation in quiescent confluent and sparse cultures of Swiss 3T3 and 3T6 cells by changing for a fresh medium containing 10-20% serum. Proliferation was inhibited by dansylcadaverine (0.1 mM), chloroquine (0.05 mM), 5-methoxytryptamine (0.1 mM), cystamine (0.1 mM), dimethylurea (100 mM), methylurea (100 mM), and in some experiments--by urea (100 mM). The inhibitory activity was not associated with a direct influence of amines on DNA synthesis or thymidine phosphorylation. The findings suggest that amines may influence the process of clustering of growth factor-receptor complexes, or the fusion of plasma membrane and intracellular vesicles containing some components required for cell proliferation.

Author List

Nikol'skiĭ NN, Barkan RS, Bogdanova NP, Vinogradova NA, Sorokin AB

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amines
Animals
Cattle
Cell Division
Cell Line
Cells, Cultured
DNA
Immune Sera
Mice
Muscles
Phosphorylation
Thymidine