Faculty Collaboration Database

Neonatal cystitis-induced colonic hypersensitivity in adult rats: a model of viscero-visceral convergence. Neurogastroenterol Motil 2011 Jul;23(7):683-e281

Date

05/20/2011

Pubmed ID

21592255

Pubmed Central ID

PMC3117950

DOI

10.1111/j.1365-2982.2011.01724.x

Scopus ID

2-s2.0-79959311098 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

BACKGROUND: The objective of this study was to determine if neonatal cystitis alters colonic sensitivity later in life and to investigate the role of peripheral mechanisms.

METHODS: Neonatal rats received intravesical zymosan, normal saline, or anesthesia only for three consecutive days [(postnatal (PN) days 14-16)]. The estrous cycle phase was determined prior to recording the visceromotor response (VMR) to colorectal distension (CRD) in adult rats. Eosinophils and mast cells were examined from colon and bladder tissues. CRD- or urinary bladder distension (UBD)-sensitive pelvic nerve afferents (PNAs) were identified and their responses to distension were examined. The relative expression of N-methyl-d-aspartic acid (NMDA)-NR1 subunit in the lumbo-sacral (L6-S1) spinal cord was examined using Western blot.

KEY RESULTS: The VMR to CRD (≥10mmHg) in the neonatal zymosan group was significantly higher than control in both the diestrus, estrus phase and in all phases combined. There was no difference in the total number of eosinophils, mast cells or number of degranulated mast cells between groups. The spontaneous firing of UBD, but not CRD-sensitive PNAs from the zymosan-treated rats was significantly higher than the saline-treated control. However, the mechanosensitive properties of PNAs to CRD or UBD were no different between groups (P>0.05). The expression of spinal NR1 subunit was significantly higher in zymosan-treated rats compared with saline-treated rats (P<0.05).

CONCLUSIONS & INFERENCES: Neonatal cystitis results in colonic hypersensitivity in adult rats without changing tissue histology or the mechanosensitive properties of CRD-sensitive PNAs. Neonatal cystitis does result in overexpression of spinal NR1 subunit in adult rats.

Author List

Miranda A, Mickle A, Schmidt J, Zhang Z, Shaker R, Banerjee B, Sengupta JN

Authors

Banani Banerjee PhD Associate Professor in the Medicine department at Medical College of Wisconsin
Adrian Miranda MD Adjunct Professor in the Pediatrics department at Medical College of Wisconsin
Jyoti N. Sengupta PhD Professor in the Medicine department at Medical College of Wisconsin
Reza Shaker MD Assoc Provost, Sr Assoc Dean, Ctr Dir, Chief, Prof in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Animals, Newborn
Colon
Colonic Diseases
Cystitis
Female
Hypersensitivity
Models, Animal
Motor Activity
Pelvis
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate
Rectum
Spinal Cord
Urinary Bladder
Zymosan