Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Toward accurate diagnosis of white matter pathology using diffusion tensor imaging. Magn Reson Med 2007 Apr;57(4):688-95



Pubmed ID




Scopus ID

2-s2.0-34248356267   314 Citations


Diffusion tensor imaging (DTI) has been widely applied to investigate injuries in the central nervous system (CNS) white matter (WM). However, the underlying pathological correlates of diffusion changes have not been adequately determined. In this study the coregistration of histological sections to MR images and a pixel-based receiver operating characteristic (ROC) analysis were used to compare the axial (lambda( parallel)) and radial (lambda( perpendicular)) diffusivities derived from DTI and histological markers of axon (phosphorylated neurofilament, SMI-31) and myelin (Luxol fast blue (LFB)) integrity, respectively, in two different patterns of injury to mouse spinal cord (SC) WM. In contusion SC injury (SCI), a decrease in lambda( parallel) matched the pattern of axonal damage with high accuracy, but lambda( perpendicular) did not match the pattern of demyelination detected by LFB. In a mouse model of multiple sclerosis (MS), lambda( perpendicular) and lambda( parallel) did not match the patterns of demyelination or axonal damage, respectively. However, a region of interest (ROI) analysis suggested that lambda( perpendicular)-detected demyelination paralleled that observed with LFB, and lambda( parallel) decreased in both regions of axonal damage and normal-appearing WM (NAWM) as visualized by SMI-31. The results suggest that directional diffusivities may reveal abnormalities that are not obvious with SMI-31 and LFB staining, depending on the type of injury.

Author List

Budde MD, Kim JH, Liang HF, Schmidt RE, Russell JH, Cross AH, Song SK


Matthew Budde PhD Associate Professor in the Neurosurgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Demyelinating Autoimmune Diseases, CNS
Diffuse Axonal Injury
Diffusion Magnetic Resonance Imaging
Disease Models, Animal
Mice, Inbred C57BL
Multiple Sclerosis
Nerve Fibers, Myelinated
ROC Curve
Statistics, Nonparametric