Faculty Collaboration Database

Influence of blood sampling site and technique on thromboxane concentrations in patients with ischemic heart disease. Am Heart J 1982 Aug;104(2 Pt 1):234-7

Date

08/01/1982

Pubmed ID

7102506

DOI

10.1016/0002-8703(82)90198-3

Scopus ID

2-s2.0-0020365527 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

Thromboxane A2 may play a role in coronary arterial spasm, unstable angina, myocardial infarction, cardiac arrhythmias, and sudden death. Although previous studies have examined peripheral, aortic, and coronary sinus concentrations of its stable metabolite, thromboxane B2 (TxB2), it is unknown, first, if blood sampling through long catheters alters the concentration of TxB2 and second, if peripheral levels of this prostanoid reflect its intracoronary production and release. In order to answer these questions, paired blood samples were obtained through an 18-gauge needle and a No. 7 or 8 French 110 to 125 cm catheter from the arterial (14 patients) and venous (16 patients) circulations; in addition, coronary sinus and peripheral venous samples were obtained in 16 patients and aortic samples were obtained in 14 of these patients. All samples were analyzed to TxB2 by radioimmunoassay. Blood sampling through long catheters did not systematically alter the concentrations of arterial TxB2 (needle, 85.5 +/- 67.5 pg/ml [mean +/- SD]; catheter, 62.3 +/- 40.9 pg/ml; p = 0.20) or venous TxB2 (needle, 182.5 +/- 170.5 pg/ml; catheter, 521.4 +/- 1536.0 pg/ml; p = 0.39). Peripheral venous TxB2 levels did not correlate with TxB2 levels in coronary sinus (r = 0.01) or the TxB2 coronary sinus/aortic ratios (r = 0.21). Thus blood sampling through long catheters across the coronary bed is both a reliable and necessary method for assessing intracoronary TxB2 production in patients with ischemic heart disease.

Author List

Hirsh PD, Firth BG, Campbell WB, Willerson JT, Hillis LD

Author

William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Aorta
Arteries
Blood Chemical Analysis
Blood Specimen Collection
Catheterization
Coronary Disease
Female
Humans
Male
Methods
Middle Aged
Radioimmunoassay
Thromboxane A2
Thromboxane B2
Thromboxanes