Faculty Collaboration Database

Effects of autonomic nerve stimulation on colorectal motility in rats. Neurogastroenterol Motil 2010 Jun;22(6):688-93

Date

01/14/2010

Pubmed ID

20067587

Pubmed Central ID

PMC2952396

DOI

10.1111/j.1365-2982.2009.01461.x

Scopus ID

2-s2.0-77951957986 (requires institutional sign-in at Scopus site)   44 Citations

Abstract

BACKGROUND: Several disease processes of the colon and rectum, including constipation and incontinence, have been associated with abnormalities of the autonomic nervous system. However, the autonomic innervation to the colon and rectum are not fully understood. The aims of this study were to investigate the effect of stimulation of vagus nerves, pelvic nerves (PN) and hypogastric nerves (HGN) on colorectal motility in rats.

METHODS: Four strain gauge transducers were implanted on the proximal colon, mid colon, distal colon and rectum to record circular muscle contractions in rats. Electrical stimulation was administered to the efferent distal ends of the cervical vagus nerve, PN and HGN. Motility index (MI) was evaluated before and during stimulation.

KEY RESULTS: Electrical stimulation (5-20 Hz) of the cervical vagus elicited significant contractions in the mid colon and distal colon, whereas less pronounced contractions were observed in the proximal colon. Pelvic nerves stimulation elicited significant contractions in the rectum as well as the mid colon and distal colon. Atropine treatment almost completely abolished the contractions induced by vagus nerve and PN stimulation. Hypogastric nerves stimulation caused relaxations in the rectum, mid colon and distal colon. The relaxations in response to HGN stimulation were abolished by propranolol.

CONCLUSIONS & INFERENCES: Vagal innervation extends to the distal colon, while the PN has projections in the distribution of the rectum through the mid colon. This suggests a pattern of dual parasympathetic innervation in the left colon. Parasympathetic fibers regulate colorectal contractions via muscarinic receptors. The HGN mainly regulates colorectal relaxations via beta-adrenoceptors.

Author List

Tong WD, Ridolfi TJ, Kosinski L, Ludwig K, Takahashi T

Authors

Kirk A. Ludwig MD Chief, Professor in the Surgery department at Medical College of Wisconsin
Timothy J. Ridolfi MD, MS, FACS Associate Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adrenergic beta-Antagonists
Animals
Autonomic Nervous System
Colon
Electric Stimulation
Gastrointestinal Motility
Hypogastric Plexus
Male
Manometry
Propranolol
Rats
Rats, Sprague-Dawley
Rectum
Vagus Nerve