Medical College of Wisconsin
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Global mapping of glycosylation pathways in human-derived cells. Dev Cell 2021 04 19;56(8):1195-1209.e7

Date

03/18/2021

Pubmed ID

33730547

Pubmed Central ID

PMC8086148

DOI

10.1016/j.devcel.2021.02.023

Scopus ID

2-s2.0-85104060411   17 Citations

Abstract

Glycans are one of the fundamental classes of macromolecules and are involved in a broad range of biological phenomena. A large variety of glycan structures can be synthesized depending on tissue or cell types and environmental changes. Here, we developed a comprehensive glycosylation mapping tool, termed GlycoMaple, to visualize and estimate glycan structures based on gene expression. We informatically selected 950 genes involved in glycosylation and its regulation. Expression profiles of these genes were mapped onto global glycan metabolic pathways to predict glycan structures, which were confirmed using glycomic analyses. Based on the predictions of N-glycan processing, we constructed 40 knockout HEK293 cell lines and analyzed the effects of gene knockout on glycan structures. Finally, the glycan structures of 64 cell lines, 37 tissues, and primary colon tumor tissues were estimated and compared using publicly available databases. Our systematic approach can accelerate glycan analyses and engineering in mammalian cells.

Author List

Huang YF, Aoki K, Akase S, Ishihara M, Liu YS, Yang G, Kizuka Y, Mizumoto S, Tiemeyer M, Gao XD, Aoki-Kinoshita KF, Fujita M

Author

Kazuhiro Aoki PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cell Line, Tumor
Gene Knockout Techniques
Glycomics
Glycosylation
HEK293 Cells
Humans
Metabolic Networks and Pathways
Polysaccharides
Reproducibility of Results